By H.D. Jakubke, J. Jeschkeit
Read or Download Amino Acids, Peptides and Proteins: An Introduction PDF
Similar introduction books
Creation to targeted Ion Beams is geared in the direction of suggestions and functions. this can be the one textual content that discusses and offers the speculation at once on the topic of purposes and the one person who discusses the sizeable functions and methods utilized in FIBs and twin platform tools.
- An Introduction to Phytoplanktons: Diversity and Ecology
- Introduction to Stellar Statistics
- 101 Things Everyone Should Know About Economics: A Down and Dirty Guide to Everything from Securities and Derivatives to Interest Rates and Hedge Funds - And What They Mean For You
- Green ink: an introduction to environmental journalism
- Introduction to Mineralogy: Crystallography and Petrology
- Introduction to Glass Science: Proceedings of a Tutorial Symposium held at the State University of New York, College of Ceramics at Alfred University, Alfred, New York, June 8–19, 1970
Additional resources for Amino Acids, Peptides and Proteins: An Introduction
In the gas chromatographic analysis of Dnp-amino acid methyl esters, amounts as small as 10-14 mol of amino acid may be detected. This extreme sensitivity is achieved with an electron capture detector, making use of the high electron affinity of the Dnp group. The separation of diastereoisomeric amino acid derivatives by gas chromatography has already been described (on p. 42). 48 Amino Acids Table 10. c: Cl.. ~a! 0 0 ~'~ '- ~ ..... ::1 \0 l!. ~ ~ '- <-J ~ E C) g I 0 Time 15 20 25 Jamin Figure 12.
This was a considerable achievement. The accuracy ( = 3 per cent) was the highest achieved up to that t-ime and very little material was used. However, the analysis of each sample took a week. MooRE and STEIN were able to perform the analysis in a shorter time by changing to ion exchange chromatography. The most suitable ion-exchangers for amino acid analysis are the Dowex resins (Dowex 50 X 8 and Dowex 50 X 4) which are crosslinked with 8 and 4 per cent divinylbenzene, respectively, and sulphonated polystyrene resins of small bead size (Amberlite resins), which \Yere used in the first automatic amino acid analysers 56 • Figure 10 illustrates the principle of the first automatic analysers developed by SPACKMANN, MooRE and STEIN.
Ultra-violet region above wavelengths of 220 nm, the aromatic amino acids tryptophan, tyrosine, phenylalanine and histidine show characteristic maxima above 250 nm. The high molar extinctions of tyrosine and tryptophan facilitate rapid quantitative determination of these amino acids in proteins. In the IR spectra of amino acids the normal NH-stretching frequencies in the region of 3300-3500 cm-1 are absent. Instead, absorption at 3070 cm- 1 6-1~ E. I ~1 3 \ ~ \ 'I\ 0zz5 245 265 285 nm315 a 2 1 I I \I I I \ ', \ ~ t( I v / ; ' \ b \ 0225 2lr5 265 285 nm 315 Figure 5.