Advances in Biochemical Engineering - Biotechnology. - by Bernhard Sonnleitner

By Bernhard Sonnleitner

With contributions by means of a number of specialists

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Additional info for Advances in Biochemical Engineering - Biotechnology. - Bioanalysis and Biosensors for Bioprocess Monitoring

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LDH activity in the supernatant can be an excellent indicator for dead or non-viable cells as demonstrated for CHO cells [343]. This method is much faster than all of the counting techniques (and is again a solution of an inverse problem). 4 Cellular Components or Activities The trick for analyzing a biocatalytic activity must also be exploited whenever the medium contains particulate matter because this, of course, would falsify any direct gravimetric determination. Herbert [165] also gives an overview of many different indirect methods.

This high number results in statistically significant data and distributions of cell properties. Therefore, flow cytometry is a key technique to segregate biomass (into distinct cell classes) and to study microbial populations and their dynamics, specifically the cell cycle [76, 87, 116, 200, 214, 221, 295, 329, 330, 409, 418]. Individual cells are aligned by means of controlled hydrodynamic flow patterns and pass the measuring cell one by one. One or more light sources, typically laser(s), are focused onto the stream of cells and a detection unit(s) measure(s) the scattered and/or fluorescent light (Fig.

G. g. g. [108, 109], O2 tension, compartmentalized redox potential, membrane potential, cell number or cell volume, see [133], and even pH. Major drawbacks are the cost of the equipment, the low intrinsic sensitivity and the interpretation of spectra [430]. Instrumentation of Biotechnological Processes 41 Fig. 26. Schematic design of field flow fractionation (FFF) analysis. A sample is transported along the flow channels by a carrier stream after injection and focusing into the injector zone. Depending on the type and strength of the perpendicular field, a separation of molecules or particles takes place: the field drives the sample components towards the so-called accumulation wall.

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